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Several is an understatement. The show probably holds some kind of record for cameo appearances.

I would argue that the Fantastic Four cartoon of the 90's had the most. Even showing X-Men who never appeared in X-Men cartoons. The show is often listed as one of the worst Marvel cartoons of that decade. I believe that has more to do with the 1st season than the highly improved upon season 2 (all new art style, adapting some of Byrne's classic 80's issues and more classic Kirby and Lee issues; better than any Marvel cartoon to date)...I mean season 2 of FF is better than Iron Man: Force Works, Avengers Assemble, Spider-Man Unlimited (Spidey in space with alien animals) and The Incredible Hulk (a few cool episodes but a lot more misses, the good episodes feature Hulk with other hero's (always loved the Sasquatch episode even though they made him full villian) and when he became Joe Fix It.
Off the top of my head here are other heroes who appeared in the Fantastic Four animated series: Daredevil (1st animated in costume appearance I believe), Hulk (double crossover from Hulk show), Thor, Iron Man, Spider-Man, Scarlet Spider (Ben O'Riley), Ghost Rider, Captain America, Black Panther, T'Chaka, Namor (+ his roster), Black Bolt and the Inhumans (first BB animated appearance as leader), She Hulk, Goliath (Clint version) Tygra, Vision, Hercules, Rage, The New Warriors (Darkhawk, Justice (Marvel Boy), Speedball), Wolverine, Cyclops, Jean, Storm, Gambit, Sabra, Juggernaut, Nova, the Watcher, the original Human Torch and OF COURSE the Silver Surfer.
Also 1 of the TMNT and The Biker Mice were shown as well.

I think that wins.